![]() Receptor-mediated targeting of fluorescent probes in living cells. (1/933)A strategy was developed to label specified sites in living cells with a wide selection of fluorescent or other probes and applied to study pH regulation in Golgi. INDIANAPOLIS The Indiana State Fair tragedy on Aug. cDNA transfection was used to target a single-chain antibody to a specified site such as an organelle lumen. The targeted antibody functioned as a high affinity receptor to trap cell-permeable hapten-fluorophore conjugates. (2011) A comparison of mean displacement values using high b-value q-space diffusion. It has the same operating habits and interface presentation as Finder, but also supports more flexible and practical features. Zareen Fatima and Ahmed Bilal Waqar-q-Space Imaging in Meningioma. Synthesized conjugates of a hapten (4-ethoxymethylene-2-phenyl-2-oxazolin-5-one, phOx) and fluorescent probes (Bodipy Fl, tetramethylrhodamine, fluorescein) were bound with high affinity (approximately 5 nM) and specific localization to the single-chain antibody expressed in the endoplasmic reticulum, Golgi, and plasma membrane of living Chinese hamster ovary cells. QSpace is a clean and efficient Multi-view File Manager. Using the pH-sensitive phOx-fluorescein conjugate and ratio imaging microscopy, pH was measured in the lumen of Golgi (pH 6.25 +/- 0.06). Measurements of pH-dependent vacuolar H+/ATPase pump activity and H+ leak in Golgi provided direct evidence that resting Golgi pH is determined by balanced leak-pump kinetics rather than the inability of the H+/ATPase to pump against an electrochemical gradient. Like expression of the green fluorescent protein, the receptor-mediated fluorophore targeting approach permits specific intracellular fluorescence labeling. These datasets are no longer available in QSpace. A significant advantage of the new approach is the ability to target chemical probes with custom-designed spectral and indicator properties. Canadian Relocation Cases: Heading Towards Guidelines, 2011. dd excitations in three-dimensional q-space: A nonresonant inelastic X-ray scattering study. ( +info)Įndocytic sorting of lipid analogues differing solely in the chemistry of their hydrophobic tails.
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